Droplet microfluidics is an emerging powerful tool for applications in biotechnology such as digital polymerase chain reaction (dPCR) or single-cell analysis. Droplets serving as microreactors are generated in microfluidic chips, enabling extremely high throughput and allowing a large number of parallel experiments to be conducted in a shorter time.
Fluorinated oils such as FO135, FO200, FO7500, FO40, and TSH7500 are commonly used as the continuous oil phase in droplet microfluidics due to their ability to dissolve and transport oxygen. Furthermore, most chemical and biochemical compounds are insoluble in fluorinated oils.
FluoSurf in FO135/FO200/FO7500/FO40 surfactant oils are biocompatible surfactant oil-phase solutions for various picoliter droplet microsphere and nanoliter droplet applications. They stabilize droplet and their cellular or molecular contents. droplet exhibit high stability across a broad range of temperatures and biological conditions, ensuring experimental reliability and reproducibility.
FluoSurf in FO135/FO200/FO7500/FO40 surfactant oil are commonly used in research projects involving monoclonal antibody discovery, cell line development, synthetic biology, stem cell engineering, isolation and analysis, single-cell genome editing (CRISPR-Cas9), single-cell disease studies, diagnostics, and prognosis.
Key Features:
1. Facilitates the generation of water-in-oil droplet for various bioassays
2. Produces stable, highly uniform droplet with diameters ranging from 1 to 300 μm
3. Ensures high-quality synthesis through rigorous quality assurance protocols
4. Ready to use
Application:
1. Cell and molecular biology
2. Analysis of cell growth and secretion
3. ESI-MS
4. Production of water-in-oil (W/O) droplet microspheres
5. Single-cell analysis and isolation
6. Digital droplet PCR
7. Screening of cells, genes, or biomolecules
8. Synthetic Biology
9. Chemical and Biological Reaction Control
10. DNA Amplification
11. 3D Cell Culture in Droplet Microspheres
12. Synthesis of Inorganic Nanoparticles
13. Alginate Microsphere Hydrogel / Polyacrylamide Microsphere / Agarose Microsphere
14. Protein Crystallization
15. Cell and Droplet Encapsulation/Sorting/Fusion/Picoinjection
16. Organoid encapsulated into droplet microspheres
17. Gene Therapy
18. GUV Vesicle Synthesis
Example of FluoSurf 2w/w% in FO7500 surfactant fluorinated oil
Example of FluoSurf 2w/w% in FO135 surfactant fluorinated oil
Example of FluoSurf 5w/w% in FO135 or FO7500 surfactant fluorinated oil
Package kit for producing droplet generation, further surfactant dilution and breaking droplet: FluoSurf 2w/w% in FO7500 surfactant fluorinated oil, FO7500 oil and Fluo-Break droplet demulsifier
FluoSurf in FO135/FO200/FO7500/FO40 surfactant oil are available in standard 2wt% and 5wt% mass concentrations by default, packaged in 10mL, 30mL, 50mL, and 100mL volumes. Additionally, we offer customized versions with concentrations such as 4wt%, 8wt%, and 10wt%.
Please provide us with the product name, item number, expected shipping date, and delivery address + contact information based on your experimental or project requirements, referencing the specifications listed in the table below. We will ship to your designated address within 14 days. Alternatively, we can arrange to ship according to your specified date to ensure convenient receipt of goods and maintain the timeliness and continuity of your experiments.
