Drop-Break, a droplet emulsion demulsifier—also known as a droplet microsphere cleaning solution or droplet disruptor—is specifically designed to disrupt water-in-oil droplet microspheres formed by fluorinated surfactants such as PFPE-PEG-PFPE, the Surf series, and the FluoSurf neat surfactant series, thereby extracting the substances inside the droplet microspheres for subsequent imaging and compositional analysis.
Instructions for Use:
1. Collect the droplet microspheres in a plastic centrifuge tube.
2. Use a pipette to remove the excess oil phase, leaving behind the droplet microsphere emulsion;
3. Add a volume of Drop-Break to the centrifuge tube equal to the volume of the droplet-microsphere emulsion. (If a high-concentration surfactant is used to produce the droplet-microspheres, the volume of Drop-Break added may be 1–2 times the volume of the droplet emulsion. Additionally, the droplet demulsification process may be repeated multiple times, such as twice or three times.)
4. Gently shake the centrifuge tube; the liquid-droplet microspheres begin to rupture;
5. Place the centrifuge tubes in the centrifuge and centrifuge for 1 minute; the droplet microspheres will completely break down, resulting in a two-phase mixture. The upper phase is the aqueous phase, and the lower phase is a mixture of the oil phase and the droplet emulsion demulsifier;
6. Use a pipette to transfer the clear supernatant from the centrifuge tube to another clean centrifuge tube. The clear supernatant will then be analyzed and characterized.
Drop-Break, a demulsifier for droplet emulsions, is a liquid and is non-toxic. Store it according to the storage conditions indicated on the bottle label.
The default packaging is 10 mL/bottle, ready to use. For other volume options, please contact us in advance for confirmation.
Order Information:
Name: Drop-Break
Item No.: TS-Drop-Break
Specifications: 10 mL/bottle; ready to use.
Unit: Bottle
Quantity: 1 or others