在发表论文、演讲报告、总结文献等情况下，有时候需要对使用的产品进行标注公司信息，以此表明数据结果是使用该公司提供的产品并通过科学的实验设计而获得的实验数据。
标注购买产品信息的方法有很多，本文针提供了若干个标注泰初科技公司信息的写法，可供您参考。同时，建议各位用户采用常见的标注方法或者便于用户阅读的标注方法。
写法一：紧随在产品后，用括号标注 公司名称+国家 或者 公司名称+公司地区+国家，例如：
HFE7500 fluorinated oil (Techu Scientific (Tianjin) CO., Ltd., China) containing 20%(V/V) PFO is used to break the droplet microspheres.
microfluidic bubble trap with PEEK material (Techu Scientific (Tianjin) CO., Ltd., China) is connected to the position bewteen reservoir and MFS3 sensor, which can remove bubbles in PBS solutions and secure the correct flow rate.
a staggered herringbone mixer (Techu Scientific (Tianjin) CO., Ltd., China) is used to produce RNA-LNP nanoparticles with size of 80 nm, two syringe pumps are used to inject water phase and RNA-LNP organic phase into mixer chip. The FRR (flow rate ratio) is from 3:1 to 9:1 and the TFR (total flow rate) is 2mL/min.
soft Tygon tubing with one meter (Techu Scientific (Tianjin) CO., Ltd., Tianjin, China) was connected to the syringes through luer fitting and 1/4-28UNF thread fitting. Soft Tygon tubing is used to dampen the pulse-flow in order to get stable liquid flow.
the glass flow-focus droplet chip with depth of 33 μm (Techu Scientific (Tianjin) CO., Ltd., Tianjin, China) was used to generate water-in-oil droplet (water droplet), prior to start experiment, we used Fluo-ST3 hydrophobic reagent (Emulseo, Pessac, France) to treat channel surface to enhance droplet generation.
写法二：紧随在产品后，用括号标注 公司名称简称+公司地区+国家，例如：
TSH7500 fluorinated oil (Techu Scientific, Tianjin, China) is used to dilute FluoSurf-C surfactant to form 2wt% concentration,
13-port manifold (Techu Scientific, Tianjin, China) is used to distribute 12-path liquid at the same time,
a portable air compressor GW206 (Techu Scientific, Tianjin, China) is used to supply gas for OB1 MK4 pressure controller (Elveflow, Paris, France),
写法三：紧随在产品后，用括号标注 货号+公司名称+国家，例如：
a flow-focus structure glass chip (SKU: TS-FFDC-GSC33, Techu Scientific (Tianjin) CO., Ltd., China) is used to produce oil-in-water droplet with diameter from 30 μm to 60 μm,
the continuous phase is Fluo-Oil 7500 oil (SKU: TS-FO7500-100) containing 2wt% of FluoSurf-S surfactant purchased from Techu Scientific (Tianjin) CO., Ltd. (China),
the water-in-oil droplet is injected into inner inlet port with a constant flow rate and TSH-7500 oil (SKU: TSH7500-100, Techu Scientific (Tianjin) CO., Ltd., China) is injected into outer inlet port which space droplet with a constant distance for accurately sorting,
写法四：紧随产品后，标注产品购买所在的公司，一种是上述三种写法中的一个方法，第二种采用 purchased from, from, obtained from, bought from, using, supplying from, supplying by, provided by 等类似词语进行引导，例如：
a flow-focus structure PDMS droplet chip is used to genetate water-in-oil droplet with diameter of 80μm, before producing droplet, Fluo-ST3 reagent purchased from Techu Scientific (Tianjin) CO., Ltd., China is used to treat channel surface to be hydrophobic which could enhance droplet generation.
two-channel pressure controller OB1 MK4 with two MFS6 sensors from Techu Scientific (Tianjin, China) is used to drive water and oil to mixer chip. Channel 1 is used to push water-phase with flow rate of 9 mL/min and channel 2 is used to drive organic-phase with flow rate of 3 mL/min. The total flow rate is 12 mL/min and flow rate ratio is 3:1.
two MPS2 small pressure sensors bought from Techu Scientific (Tianjin) CO., Ltd. (China) are used to measure pressure of inlet port and outlet port on a straight PDMS chip, then calculate pressure difference. one MUX Distribution12 valve (SKU: MUX-D-12, Techu Scientific (Tianjin) CO., Ltd., China) is used to inject ten different samples into PDMS chip based on the desired sequence.
写法五：有时为了便于用户阅读，在描述实验过程或采用几乎同质化的产品时，可采用产品通用名称进行描述，紧随在产品名称后，标注产品名称或货号+公司名称+国家 或者 产品名称或货号+公司名称+公司地区+国家。
in order to get eight different multi-color droplet automatically, a 12-way rotary valve (MUX Distribution12, Techu Scientific (Tianjin) CO., Ltd., China) was used to distribute eight different dispersed phase to inner inlet port of PDMS droplet chip. Continuous oil phase (Surf-DG7500 Pro droplet generation oil, Techu Scientific (Tianjin) CO., Ltd., China) was used to stable droplet and encapsulated cells. High-performance pressure controller (OB1 MK4, Elveflow, France) was used to inject continous phase and dispersion phase into droplet chip. Two microfluidic flow sensors (MFS3 sensor, Elveflow, France) was used to monitor the real-time flow rate or control constant flow rate through advanced PID method. The sequence module of ESI software (Elveflow, France) was used to control the operation of the whole experiment. After passing five hours, the resulting production was collected and then further analyzed.
Staggered herringbone micromixer (SKU: TS-SHM-GCC, Techu Scientific, Tianjin, China) was used as the microfluidic device to prepare liposomes. Lipids were dissolved in ethanol to prepare lipid mixture of appropriate concentration and molar ratio. Aqueous media was used to prepare the liposomes included deionised water (DW), isotonic normal saline, phosphate buffered saline, HEPES buffered saline and ammonium sulfate. All aqueous media were passed through 0.22 μm polyether sulfone syringe filter before injecting into the micromixer. Ethanol solution of lipid mixture and aqueous medium were injected into the micromixer with the volume flow rates controlled by two syringe pumps (ProSense, Oosterhout, Netherlands) using AMSPro software. The flow rate ratio (FRR) bewteen the aqueous and the organic phase was varied from 2:1 to 5:1 and the total flow rate (TFR), defined as the sum of the aqueous flow rate and the organic flow rate, was also varied from 200 μL/min to 900 μL/min in order to determine the effect of flow conditions on particle size and ploydispersity index (PDI). Liposomes were collected from the outlet of the micromixer and ethanol was removed by dialysis overnight at room temperature, against 1 L of the same aqueous medium used in the preparation, under constant stirring.
Two-port reservoir (cat# Tank15-P2QC, Techu Scientific (Tianjin) CO., Ltd., Tianjin, China) were used to conenct to peristaltic pump, the side port of two-port reservoir was connected to a 0.22 μm filter valve to filter the air. Peristaltic pump was used to control flow rate of 2 mL/min and injected cell culture medium into microfluidic chip.
参考示例如下：
Cells were grown to a density of 1.2–3×10^6 cells/mL in prior to each experiment. The cell suspensions were further diluted to a value corresponding to 0.175 cells/drop in each of the wanted drop sizes. Upon drop formation, cell suspensions were emulsified using Surf-DG7500 droplet generation oil (Techu Scientific, Tianjin, China). Cell suspension and Surf-DG7500 droplet generation oil were separately loaded into 1 mL Omnifix-F syringes (B Braun) with 0.4× 19 mm needle tips and pumped into the drop making device by low pressure neMESYS syringe pumps (CETONI GmbH) with flow rates corresponding to the wanted drop sizes. For 0.2 nL drops and 0.5nL drops the flow rates of the fluorinated oil were 300 µL/h and 850 µL/h and for the cell suspension 280 ± 10 µL/h and 250 ± 20 µL/h , respectively. Syringes were connected with microfluidic devices by PTFE tubing in dimensions of 0.3 mm inner diameter and 0.76 mm outer diameter. After drop making, the emulsion was collected off-chip in an incubation chamber or a collection vial. After collection, the emulsion-containing collection device was incubated at 37◦ C with 5% CO2 until further use. All cell counting were performed by eye directly from the incubation chamber containing the emulsified cells during a time span of 30–45 min. For all drop and cell counting upon fixation the in incubation chamber, a microscope incubator (Okolab) was used set at 37◦C.
To produce the water-in-oil monodispersed emulsions in the microfluidic chips, we used Surf-DG7500 Pro droplet generation oil (Techu Scientific (Tianjin) CO., Ltd., China) as a continuous phase. MilliQ deionized water (DI) was used as a dispersed phase. An optical microscope (Axiovert 200, Zeiss, Germany) with a camera was used for the visual control of the droplet-generation process and for measurements of the droplet diameters and generation frequencies. They were performed manually using the captured video frames in ImageJ software.
Prior to the micro-droplet generation experiments, all channels of the fabricated microfluidic devices were coated with Fluo-ST3 reagent (Emulseo, France) to make their surfaces hydrophobic. It is known that the hydrophobic surface promotes the stable micro-droplet generation. While the sample solution (disperse phase) flowed into the middle channel, one of two sample inlets was clamped. The oil solution (continuous phase) was mixed with a surfactant (003 Surfactant, Techu Scientific, Tianjin, China) at 2% (w/w) in HFE-7500 oil for stable micro-droplet generation and storage. Both the sample and oil solutions were injected using syringe pumps (LEGATO 111, KD Scientific Inc., Holliston, MA, USA). Micro-droplet generation was monitored using an optical microscope equipped with a high-speed camera (VEO E310L, Phantom, Wayne, NJ, USA). The generated micro-droplets were collected through the outlet, and their sizes were measured with bright-field microscopy and a ImageJ software.
A 2-inlet flow focusing device was prepared using standard soft-lithography techniques. Briefly, the microfluidic mold was obtained by coating a 4-inch silicon wafer with SU-8 photoresist (MicroChem Corp.) reticulated upon UV exposure. Following careful cleaning of the mold with isopropanol, a 10:1 mixture of Sylgard 184 PDMS resin/curing agent (Dow Corning) was poured onto the mold, degassed under vacuum and baked for 2 hours at 70°C. The PDMS slab was piled off the mold and inlets and outlets were punched with a 1.5 mm diameter biopsy puncher. The PDMS slab was bound on a 1 mm thick glass slide immediately following oxygen plasma activation. The chip channel was treated to be hydrophobic using Fluo-ST3 reagent (Emulseo, France). Monodisperse water-in-oil droplets were generated by mixing the aqueous samples and the continuous phase (fluorinated oil HFE-7500 + 2wt% 003 surfactant, Techu Scientific (Tianjin) CO., Ltd., China) on chip using a pressure pump controller OB1 MK4 (Elveflow, France) and 200 µm inner diameter PTFE tubing.
A cell suspension is coencapsulated in drops at the single-cell level and the reagent mix on a coflow drop maker design. Droplets of 60 pL are generated with fluorinated oil (Novec7500, 3M) containing 1% of Fluosurf-O surfactant from Techu Scientific (Tianjin) CO., Ltd. (China). Once formed, the drops are squeezed all together by an extraction of oil before being incubated into 70 μm deep delay lines (incubation chamber). The fluorophores in the droplets are excited with a laser (λlaser = 532 nm) at a 20 μm deep constriction where drops are passing one by one allowing the detection of the increase of fluorescence at different positions along the incubation chambers line.
As for droplet generation and cell encapsulation, key reagents included Fluo-ST3 (Emulseo, France) for hydrophobic treatments of microfluidic channels, FluoSurf 2% in Fluo-Oil 135 (SKU: TS-FSC-FO135-210) purchased from Techu Scientific (Tianjin) CO., Ltd., China as the oil phase for K562 cells or A549 and HeLa cells in droplet generation, respectively, iodixanol (OptiPrep™ Density Gradient Medium, Sigma-Aldrich Corp., USA, cat: D1556) and stripping buffer (Thermo Scientific Corp., USA, cat: 62300) as two key supplements in the water phase of droplet generation to match solution densities and lyse cells, respectively. More specifically, for K562 cells or A549 and HeLa cells, iodixanol was supplemented with volume ratios of 12% or 30%, respectively. When iodixanol was taken into consideration, the maximum concentrations of stripping buffers in cell suspensions were calculated as 2 × and 1.25 × for K562 cells or A549 and HeLa cells, respectively, under the condition of supplementing the 5 × stripping buffer (stock solution).